Table 1 Criteria for laboratory evaluation
From: The Brazilian Network for HIV-1 Genotyping External Quality Control Assurance Programme
| Sample 1 | Sample 2 | |||
|---|---|---|---|---|
| Qualitative variables | ||||
| Positive PCR amplification | ||||
| 15 | 15 | 30 | ||
| Genetic diversity of sequenced samples | ||||
| Less than 2.2% | 10 | 10 | 20 | |
| Quantitative variables | ||||
| Number of obtained sequences | ||||
| 4 | 2 | 2 | ||
| 5 | 4 | 4 | ||
| 6 | 8 | 8 | ||
| 7 | 10 | 10 | 20 | |
| Correct interpretation of mutations (codons) | ||||
| Protease 1 | 1 | 1 | ||
| 2 | 1 | 1 | ||
| 3 | 1 | 1 | ||
| 4 | 1 | 1 | ||
| 5 | 1 | 1 | 10 | |
| Reverse transcriptase 1 | 1 | 1 | ||
| 2 | 1 | 1 | ||
| 3 | 1 | 1 | ||
| 4 | 1 | 1 | ||
| 5 | 1 | 1 | 10 | |
| Quality of sequences (Phred quality scores) | ||||
| 100% above 30 | 5 | 5 | ||
| 80-99% above 30 | 2.5 | 2.5 | 10 | |
| Total | 100 | |||